李璐

职称：

教授

专业：

分析化学

博（硕）导：

博导

最终学历：

博士研究生

联系电话：

15954911357

电子邮件：

lilu5252@163.com

简历及研究领域

个人简介：

李璐，女，汉族，教授，博士生导师。2005年与2010年于山东大学分别获得学士学位和博士学位，2011年9月入职山东师范大学工作至今，2017年晋升教授。目前主要从事生物分子的单细胞、单分子分析研究，在Chem. Sci.,   Anal. Chem.,Chem. Commun.等杂志上发表SCI论文40余篇，其中以第一作者或通讯作者发表论文20余篇，主持多项国家自然科学基金重大研究计划培育项目、面上基金等多项国家级和省部级项目。2018年获得山东省高等学校科学技术一等奖1项（第一位）及山东省优秀硕士学位论文指导教师称号。获2019年山东省杰出青年基金，并入选山东省泰山学者青年专家，

代表性论文

1. Haimeng Pei,† Lu Li,*,† Yuan Wang,‡ Renjie Sheng,† Yiguo Wang,§ Shuting   Xie, Lingling Shui,Haibin Si,† and Bo Tang*,†. Single-Cell Phenotypic   Profiling of CTCs in Whole Blood Using anIntegrated Microfluidic Device. Anal.Chem., 2019, 91, 11078−11084.

2. Yuanyuan Fan, Lu Li,* Meng Lu, Haibin Si and Bo Tang*. In situ   fluorescent profiling of living cell membrane proteins at a single-molecule   level. Chem. Commun., 2019, 55, 4043-4046.

3. Lu Li, Meng Lu, Yuanyuan Fan, Lingling Shui, Shuting Xie, Renjie Sheng, Haibin   Si, Qingling Li, Yiguo Wang and Bo Tang *. High-throughput and   ultra-sensitive single-cell profiling of multiple microRNAs and   identification of human cancer. Chem. Commun., 2019, 55, 10404-10407.

4. Qingling Li, Wei Li, Shuang Cui,Qianqian Sun, Haibin Si, Zhenzhen Chen,   Kehua Xu,Lu Li⁎, Bo Tang⁎. Dynamic fluorescent   imaging analysis of mitochondrial redox in single cells with a microfluidic   device. Biosen. Bioelectro., 2019,129,132–138

5. Yuanyuan Fan,† Defang Dong,† Qingling Li, Haibin Si, Haimeng Pei,Lu Li*   and Bo Tang*. Fluorescent analysis of   bioactive molecules in single cells based on microfluidic chips. Lab Chip, 2018, 18, 1151-1173.

6. Haibin Si, Renjie Sheng, Qingling Li, Jie Feng, Lu Li*, and Bo Tang*.Highly   Sensitive Fluorescence Imaging of Zn²⁺ and Cu²⁺ in   Living Cells with Signal Amplification based on Functional DNA SelfAssembly. Anal.Chem., 2018, 90, 8785–8792.

7. Haibin Si, Lijuan Wang, Qingling Li, Xiaoxiao Li, Lu Li* and Bo Tang*. In-situ   fluorescent monitoring of diagnosis and treatment: a versatile nanoprobe   combining tumor targeting based on MUC1 and controllable DOX release by   telomerase. Chem. Commun., 2018, 54, 8277-8280.

8. Lu Li, Yuanyuan Fan, Qingling Li, Renjie Sheng, Haibin Si, Juan Fang,   Lili Tong, and Bo Tang*. Simultaneous Single-Cell Analysis of Na⁺,   K⁺, Ca²⁺, and Mg²⁺ in Neuron-Like PC-12   Cells in a Microfluidic System. Anal. Chem., 2017, 89, 4559−4565.

9. Lu Li, Jie Feng, Haiyun Liu, Qingling Li, Lili Tong, Bo Tang*. Two-color   imaging of microRNA with enzyme-free signal amplification via hybridization   chain reactions in living cells. Chem.   Sci., 2016, 7, 1940-1945.

10. Lu Li^#, Qingling Li^#, Peilin Chen, Zhongyi Li,   Zhenzhen Chen, Bo Tang*. Consecutive Gated Injection-Based Microchip   Electrophoresis for Simultaneous Quantitation of Superoxide Anion and Nitric   Oxide in Single PC-12 Cells. Anal.   Chem., 2016, 88, 930-936.

11. Qingling Li, Peilin Chen, Yuanyuan Fan, Xu Wang, Kehua Xu, Lu Li,* and Bo   Tang*.Multicolor Fluorescence Detection-Based Microfluidic Device for   Single-Cell Metabolomics: Simultaneous Quantitation of Multiple Small   Molecules in Primary Liver Cells. Anal. Chem., 2016, 88, 8610−8616.

12. Lu Li, Jie Feng, Yuanyuan Fan, Bo Tang*. Simultaneous Imaging of Zn²⁺   and Cu²⁺ in Living Cells Based on DNAzyme Modified Gold   Nanoparticle. Anal. Chem., 2015, 87,   4829–4835.

13. Lu Li^#, Ping Li^#, Juan Fang, Qingling Li, Haibin   Xiao, Hui Zhou, Bo Tang*. Simultaneous Quantitation of Na⁺ and K⁺   in Single Normal and Cancer Cells Using a New Near-Infrared Fluorescent   Probe. Anal. Chem., 2015, 87,   6057-6063.

14. Lu Li, Xiuli Wang, Qingling Li, Pengyuan Liu, Kehua Xu, Hao Chen*, Bo   Tang*. An Accurate Mass Spectrometric Approach for the Simultaneous   Comparison of GSH, Cys, and Hcy in L02 Cells and HepG2 Cells using New NPSP   Isotope Probes. Chem. Commun.,   2015, 51, 11317-11320.

15. Li Lu, Shen Liping,Zhang, Xiaoqian,Shui Lingling,Sui Benhui,Zhang Xiaoli,Zhao   Xiaofan,Jin Wenrui*.Multiplexed optical coding nanobeads and their   application in singlemolecule counting analysis for multiple gene expression   analysis. Analytica Chimica Acta . 2015,886,123-132.

16. Lu Li，Xianwei Wang，Xiaoli Zhang，Jinxing Wang，Wenrui Jin*.Single-cell   multiple gene expression analysis based on single-molecule-detection   microarray assay for multi-DNA determination. Analytica Chimica Acta , 2015, 854,122–128.

17. Lu Li, Qian Wang, Jie Feng, Lili Tong, Bo Tang*. Highly Sensitive and   Homogeneous Detection of Membrane Protein on a Single Living Cell by Aptamer   and Nicking Enzyme Assisted Signal Amplification Based on Microfluidic   Droplets. Anal. Chem., 2014, 86,   5101-5107.

18. Haiyun Liu^#, Lu Li^#, Qian Wang, Lili Duan, Bo   Tang*. Graphene Fluorescence Switch-Based Cooperative Amplification: A   Sensitive and Accurate Method to Detection MicroRNA. Anal. Chem., 2014, 86, 5487-5493.

19. Ruirui Zhang^#, Lu Li^#, Jie Feng, Lili Tong, Qian   Wang, Bo Tang*. Versatile Triggered Release of Multiple Molecules from   Cyclodextrin Modified Gold Gated Mesoporous Silica Nanocontainers. ACS Appl. Mater. Interfaces, 2014, 6,   9932-9936.

20. Lu Li, Feifei Gao, Jian Ye, Zhenzhen Chen, Qingling Li, Wen Gao, Lifei   Ji, Ruirui Zhang, Bo Tang*. A FRET-based Bio-friendly Apo-GOx modified Gold   Nanoprobe for Specific and Sensitive Glucose Sensing and Cellular Imaging. Anal. Chem., 2013, 85, 9721-9727.

21. Haiyun Liu^#, Lu Li^#, Lili Duan, Xu Wang, Yanxia   Xie, Lili Tong, Qian Wang, Bo Tang*.   High Specific and Ultrasensitive Isothermal Detection of MicroRNA by Padlock   Probe-Based Exponential Rolling Circle Amplification. Anal. Chem., 2013, 85, 7941-7947.

22. Lu Li, Xiaofei Hu, Yuming Sun, Xiaoli Zhang⁎, Wenrui Jin⁎.Electrochemiluminescence   resonance energy transfer between quantum dots (QDs) as the donor and Cy5 dye   molecules as the acceptor in QD-Cy5 conjugates with biomolecules as the   linker. Electrochem. Commun., 2011, 13, 1174–1177.

23. Lu Li,Xun Qu,Jintang Sun,Meixiang Yang,Bingfeng Song,Qianqian Shao,Xiaoli   Zhang,Wenrui Jin∗.Single-molecule-counting   protein microarray assay with nanoliter samples and its application in the   dynamic protein expression of living cells. Biosen. Bioelectro., 2011, 26,3688–3691.

24. Lu Li,Xiaofei Hu,Yuming Sun,Xiaoli Zhang⁎, Wenrui Jin⁎.Electrochemiluminescence   resonance energy transfer between an emitter electrochemically generated by   luminol as the donor and luminescent quantum dots as the acceptor and its   biological application. Chem. Commun., 2011, 47, 8292–8294

25. Lu Li, Xinzhe Tian,† Guizheng Zou, Zhikun Shi, Xiaoli Zhang,* and Wenrui   Jin*. Quantitative Counting of Single Fluorescent Molecules by Combined   Electrochemical Adsorption Accumulation and Total Internal Reflection   Fluorescence Microscopy. Anal. Chem. , 2008, 80, 3999–4006.

研究课题

循环肿瘤细胞上皮间质转化表型的单细胞荧光/拉曼双模态可视化分析，2019.01- 2021.12，70万元，主持

国家自然科学基金重大研究计划培育项目

基于信号放大的肺癌、肝癌细胞内多种活性分子同时高灵敏荧光成像新方法研究，2017.01-2020.12，65万元，主持

国家自然科学基金面上项目

肿瘤液体活检中的单细胞和单分子分析，2019.07-2022.06，60万元，主持

山东省杰出青年基金项目

肿瘤细胞内多种生物分子的同时单细胞高灵敏荧光成像和定量研究，2016.11-2019.11，30万元，主持

山东省自然科学基金省属高校优秀青年人才联合基金

荣誉称号

山东省泰山学者青年专家

山东省优秀硕士学位论文指导教师

山东省杰出青年基金获得者